Biological Macromolecule Crystallization Database (version 7.0)
1BG0
Crystal
Title: TRANSITION STATE STRUCTURE OF ARGININE KINASE
Description: ARGININE KINASE, ADENOSINE-5'-DIPHOSPHATE, D-ARGININE
Structure weight: 40869.287 Resolution: 1.860
Density Matthews: 2.240 Density % solution: 45.000 Z value: 4
| Length | Angles |
|---|---|
| a = 65.439 | alpha = 90.000 |
| b = 70.885 | beta = 90.000 |
| c = 80.437 | gamma = 90.000 |
Space group: P 21 21 21
Deposition Date: 1998-06-03
Crystallization Methods
Temperature:
pH: 7.5
Crystallization method:
Details: PROTEIN WAS CRYSTALLIZED FROM 18% PEG 6000, 53 MM MGCL2, 2MM MGADP-, 25 MM KNO3, 10 MM ARGININE 0.5 MM DTT, 2.5 MM SODIUM AZIDE, 25 MM HEPES, PH 7.5, WITH 20 MG/ML OF PROTEIN CONCENTRATION.
pH: 7.5
Crystallization method:
Details: PROTEIN WAS CRYSTALLIZED FROM 18% PEG 6000, 53 MM MGCL2, 2MM MGADP-, 25 MM KNO3, 10 MM ARGININE 0.5 MM DTT, 2.5 MM SODIUM AZIDE, 25 MM HEPES, PH 7.5, WITH 20 MG/ML OF PROTEIN CONCENTRATION.
Crystallogenic Reagents
| Chemical | Concentration1 | Concentration2 | Concentration units | pH1 | pH2 |
|---|---|---|---|---|---|
| PEG 6000 | 18 | % | |||
| MGCL2 | 53 | MM | |||
| MGADP | 2 | MM | |||
| KNO3 | 25 | MM | |||
| ARGININE | 10 | MM | |||
| DTT | 0.5 | MM | |||
| SODIUM AZIDE | 2.5 | MM | |||
| HEPES | 25 | MM | 7.500 | ||
| PROTEIN CONCENTRATION | 20 | MG/ML |
Polymers
Weight: 40118.562 No. of molecules: 1
E.C. number: 2.7.3.3 Mutation:
Source method: man Scientific name: Limulus polyphemus Common name: Atlantic horseshoe crab
Sequence type: polypeptide(L) Length: 360 Isoelectric Point: 6.35
Sequence:
VDQATLDKLEAGFKKLQEASDCKSLLKKHLTKDVFDSIKNKKTGMGATLLDVIQSGVENLDSGVGIYAPDAESYRTFGPL
FDPIIDDYHGGFKLTDKHPPKQWGDINTLVGLDPAGQFIISTRVRCGRSLQGYPFNPCLTAEQYKEMEEKVSSTLSSME
D ELKGTYYPLTGMSKATQQQLIDDHFLFKEGDRFLQTANACRYWPTGRGIFHNDAKTFLVWVNEEDHLRIISMQKGGDL
KT VYKRLVTAVDNIESKLPFSHDDRFGFLTFCPTNLGTTMRASVHIQLPKLAKDRKVLEDIASKFNLQVRGTRGEHTES
EGG VYDISNKRRLGLTEYQAVREMQDGILEMIKMEKAAA
Ligands
Weight: 62.005 No. of molecules: 2
Source method: syn
Weight: 24.305 No. of molecules: 1
Source method: syn
Weight: 427.201 No. of molecules: 1
Source method: syn
Weight: 175.209 No. of molecules: 1
Source method: syn
Weight: 18.015 No. of molecules: 298
Source method: nat
Literature
Blanc, E., Chapman, M.S., Ellington, W.R., Parthasarathy, G., Somasundaram, T., Zhou, G.
Blanc, E., Chapman, M.S., Chen, Z., Somasundaram, T., Zhou, G.
Ables, A., Chapman, M.S., Ellington, W.R., Parthasarathy, G., Roy, L., Somasundaram, T., Strong, S.J., Zhou, G.
PubMed references: